4. trénink v Assenu: Quartararo, Viňáles, Rins
| P. | Jezdec | # | Tým | Motocykl | Km/h | Čas | Ztráta na 1./předch. | |
| 1. |  |
Fabio Quartararo | 20 | Petronas Yamaha SRT | Yamaha | 305,2 | 1:32,952 | - |
| 2. |  |
Maverick Viñáles | 12 | Monster Energy Yamaha MotoGP | Yamaha | 309,3 | 1:33,093 | 0,141 / 0,141 |
| 3. | |
Álex Rins | 42 | Team SUZUKI ECSTAR | Suzuki | 311,2 | 1:33,262 | 0,310 / 0,169 |
| 4. | |
Marc Márquez | 93 | Repsol Honda Team | Honda | 312,7 | 1:33,268 | 0,316 / 0,006 |
| 5. |  |
Andrea Dovizioso | 4 | Ducati Team | Ducati | 315,0 | 1:33,801 | 0,849 / 0,533 |
| 6. | |
Joan Mir | 36 | Team SUZUKI ECSTAR | Suzuki | 309,1 | 1:33,849 | 0,897 / 0,048 |
| 7. | |
Danilo Petrucci | 9 | Ducati Team | Ducati | 311,5 | 1:33,981 | 1,029 / 0,132 |
| 8. |  |
Takaaki Nakagami | 30 | LCR Honda IDEMITSU | Honda | 308,0 | 1:34,069 | 1,117 / 0,088 |
| 9. | |
Pol Espargaró | 44 | Red Bull KTM Factory Racing | KTM | 310,9 | 1:34,133 | 1,181 / 0,064 |
| 10. | |
Franco Morbidelli | 21 | Petronas Yamaha SRT | Yamaha | 305,1 | 1:34,195 | 1,243 / 0,062 |
| 11. |  |
Jack Miller | 43 | Pramac Racing | Ducati | 313,5 | 1:34,209 | 1,257 / 0,014 |
| 12. | |
Andrea Iannone | 29 | Aprilia Racing Team Gresini | Aprilia | 306,9 | 1:34,233 | 1,281 / 0,024 |
| 13. | |
Francesco Bagnaia | 63 | Pramac Racing | Ducati | 307,7 | 1:34,240 | 1,288 / 0,007 |
| 14. | |
Aleix Espargaró | 41 | Aprilia Racing Team Gresini | Aprilia | 308,3 | 1:34,347 | 1,395 / 0,107 |
| 15. | |
Johann Zarco | 5 | Red Bull KTM Factory Racing | KTM | 307,4 | 1:34,370 | 1,418 / 0,023 |
| 16. |  |
Miguel Oliveira | 88 | Red Bull KTM Tech 3 | KTM | 304,8 | 1:34,465 | 1,513 / 0,095 |
| 17. |  |
Cal Crutchlow | 35 | LCR Honda CASTROL | Honda | 313,6 | 1:34,547 | 1,595 / 0,082 |
| 18. | |
Valentino Rossi | 46 | Monster Energy Yamaha MotoGP | Yamaha | 303,8 | 1:34,620 | 1,668 / 0,073 |
| 19. |  |
Karel Abraham | 17 | Reale Avintia Racing | Ducati | 306,9 | 1:34,838 | 1,886 / 0,218 |
| 20. |  |
Hafizh Syahrin | 55 | Red Bull KTM Tech 3 | KTM | 306,9 | 1:35,304 | 2,352 / 0,466 |
| 21. | |
Esteve Rabat | 53 | Reale Avintia Racing | Ducati | 309,3 | 1:35,648 | 2,696 / 0,344 |
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Marc Márquez (Repsol Honda) získal 12. vítězství letošní sezony na Gran Premio Motul de la Comunitat Valenciana, Jorge Lorenzo ve svém posledním závodě MotoGP získal body a pomohl tak týmu získat titul. Na pódium se dostali Fabio Quartararo (Petronas Yamaha SRT) a Jack Miller (Pramac Racing).
